LOCUS       sequence-67896-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-34
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             429..437
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(509..533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttt catttcagcc
      121 actgcagatg tccaacaaac aactctaaat ctccccgtaa ctatgcgaca ggccacagct
      181 tccttttttt tcccattcaa tactttctcg acgttattca ataactacta ataccactac
      241 tgactattat tcttataacg gacaatgctc gagaggcagt gtggttagct ggttgcatat
      301 ttccttgaca acggctacct tcactgccac cccgaacatg tcgtccatct ttgaacactg
      361 gtgagtgaaa gtttcttttc ggtttcatct tagatcagaa accttgagtt tttgaaaagt
      421 tgaggaggta aggaggtaaa cagaaaaaaa acgtgtggcg ttcaggtagc agagcgaaac
      481 aaaacgaaaa agagaaggaa aagatcaaac ccagctttct tgtacaaagt ggttgatcta
      541 gagggcccgc agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat
      601 gcagactgcc tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat
      661 ggaatgtaaa gaagtatgta tctcaggccg ggacctctct cgccgcactg aggggcactc
      721 cacaccacgg gggccgccgc ggccgacagc cagccaggcg gctgtggtgg gggtgaggct
      781 ggggcggcag gctgcctggg cgttggtgga cggtcgtgtc cccgccagca ttgttctgtc
      841 actttgcctg tgtcagcagg tggaagtgct caccagacat tcgaaggtaa gcctatccct
      901 aaccc
//
