LOCUS       sequence-67897-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-40
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(428..452)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             805..846
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca tatgccagct
      121 gatttcgtct tggtaataag ctcgtcattg agattatcac cgggtgtaaa tcagcttggc
      181 tctggtgtct ccgaacctcc tgtccgcacc tcagtggatg tatgccatga tgataagata
      241 tcagaaatcc tatcaccggg tgtacatcag ctaaggtgcg ggtacaggtg catttgatat
      301 caaggtgagt tatttttaag tgggtcccag agaccttggt ggtttttctc tgcagtgata
      361 gatacttcta acaactcgct atcaccgggt gaaaaatcac ctaggtctgg agcctcctgc
      421 taagcttacc cagctttctt gtacaaagtg gttgatctag agggcccgca gtggggtcag
      481 cttctaccgg ggcggcgtcc cggggtcttg gaactgcatg cagactgcct gcttgggaaa
      541 catacttctt tatatgccca tatggacctg ctaagctatg gaatgtaaag aagtatgtat
      601 ctcaggccgg gacctctctc gccgcactga ggggcactcc acaccacggg ggccgccgcg
      661 gccgacagcc agccaggcgg ctgtggtggg ggtgaggctg gggcggcagg ctgcctgggc
      721 gttggtggac ggtcgtgtcc ccgccagcat tgttctgtca ctttgcctgt gtcagcaggt
      781 ggaagtgctc accagacatt cgaaggtaag cctatcccta accctctcct cggtctcgat
      841 tctacgcgta ccggttagta atgagtttaa acgggggagg ctaactgaaa cac
//
