LOCUS       sequence-67898-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-43-44 context
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(510..534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctct tcttcaccgg
      121 gttaacatct acagaggtcc aaaaaggggt aactattggc actagtcgcc cgtgacatca
      181 agaaactagt gattatgcca aaccacaggg acatatcaca gtttacttgc tgtcgcgggc
      241 ggtgctgagt ttaaggcctc tgaaaagaga aaatggccaa tctggatgtg ctcgttggtc
      301 atagacgtca acacgaactg ttcatatgac tagagacaca ttcagcttgg cctgcttctc
      361 tagttttgaa tttttcaaca gtaagtgatt aactaacaaa acttagatgt ttacatatta
      421 agtattaaaa atatcttcgg ttttggaagg gtaagttttg ataactttta gatccataga
      481 gatatgaccg ctcaagattc aaaacaatga cccagctttc ttgtacaaag tggttgatct
      541 agagggcccg cagtggggtc agcttctacc ggggcggcgt cccggggtct tggaactgca
      601 tgcagactgc ctgcttggga aacatacttc tttatatgcc catatggacc tgctaagcta
      661 tggaatgtaa agaagtatgt atctcaggcc gggacctctc tcgccgcact gaggggcact
      721 ccacaccacg ggggccgccg cggccgacag ccagccaggc ggctgtggtg ggggtga
//
