LOCUS       sequence-67901-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter dme-mir-34
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(510..534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctaa ctgaagttgg
      121 tcgcaaaaca tccgcaagat ggtgaagcta aagcgaattt ggcttctgca caagatattc
      181 aaaagtaacc gttacacacg actattctca atcaaggaga attaaccaaa ttagtgaatt
      241 aatccaaaca agtattctta attggctatg cgctttggca gtgtggttag ctggttgtgt
      301 agccaattat tgccgttgac aattcacagc cactatcttc actgccgccg cgacaagcta
      361 aagcaactta taaaggacat ggcggaggaa ctggctcata atcacatcgg attgtctgga
      421 atctcgtcta gcactaggaa agattttaga aaatgtagga cctgtatcag atggttatgg
      481 cagtagttat catatctata atctattaaa cccagctttc ttgtacaaag tggttgatct
      541 agagggcccg cagtggggtc agcttctacc ggggcggcgt cccggggtct tggaactgca
      601 tgcagactgc ctgcttggga aacatacttc tttatatgcc catatggacc tgctaagcta
      661 tggaatgtaa agaagtatgt atctcaggcc gggacctctc tcgccgcact gaggggcact
      721 ccacaccacg ggggccgccg cggccgacag cc
//
