LOCUS       sequence-67902-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter dme-mir-92a
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(504..528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttt gaaattcgag
      121 atttttgggc tttggtcgat gctcctattg ttcgcctacc tgcctacctg ttgatggtat
      181 tatttgtgaa tgtttatcaa gatgttccat gtacttgctg aaactcaata ttcgtgatct
      241 gattttcagc cgaatataaa tatgaatttc ccgtaggacg ggaaggtgtc aacgttttgc
      301 atttcgaata aacattgcac ttgtcccggc ctatgggcgg tttgtaataa acaactaaaa
      361 tctataaaat ttatttatta ttactaaagt catgtctcgt atttttgctt atgaaatatt
      421 ttctgttgtt tcagatctcg aattcgcaat atcagaacat ctgtgttatt ttaattatat
      481 aaaagtatat atacaggtga gttacccagc tttcttgtac aaagtggttg atctagaggg
      541 cccgcagtgg ggtcagcttc taccggggcg gcgtcccggg gtcttggaac tgcatgcaga
      601 ctgcctgctt gggaaacata cttctttata tgcccatatg gacctgctaa gctatggaat
      661 gtaaagaagt atgtatctca ggccgggacc
//
