LOCUS       sequence-67903-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter dme-mir-125
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(514..538)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctac ccaatcccca
      121 gccatacaaa agttggtggt gcaacaaatt ttgattgtgg acaacaaaga aagtgttctg
      181 agccaaacta ttgtttaata tcattacgaa tgccaaagta tgtaaatgca accgggcata
      241 tgtaaatatt ggcattggtg acatgtgcaa atgtttgtat ggctgattcc ctgagaccct
      301 aacttgtgac ttttaatacc agtttcacaa gttttgatct ccggtattgg acgcaaactt
      361 gctgatgtta gtaaaaaata aggcaaaaac gaattccctt tcaaggcaca agtatcggca
      421 attcagagaa gttctccaga aggtaaaagc tcacacaaaa aatataacaa gctgtatgcc
      481 ctaagattaa agtttcttca acagaaatat gccacccagc tttcttgtac aaagtggttg
      541 atctagaggg cccgcagtgg ggtcagcttc taccggggcg gcgtcccggg gtcttggaac
      601 tgcatgcaga ctgcctgctt gggaaacata cttctttata tgcccatatg gacctgctaa
      661 gctatggaat gtaaagaagt atgtatctca ggccgggacc tctctcgccg cactgagggg
      721 cactccacac cacggggg
//
