LOCUS       sequence-67911-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-let-7a-1
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(513..537)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             890..931
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag ttaagctatc aacaagtttg tacaaaaaag
      121 caggctcatt ttcaacgtaa gtgaatgaaa atggtctaat actatttttc ttatcactca
      181 cacaggaaac caggattacc gaggaggaaa aaaagccttc ctgtggtgct caactgtgat
      241 tccttttcac cattcaccct ggatgttctc ttcactgtgg gatgaggtag taggttgtat
      301 agttttaggg tcacacccac cactgggaga taactataca atctactgtc tttcctaacg
      361 tgatagaaaa gtctgcatcc aggcggtctg atagaaagtc agttaactaa ttgtacaata
      421 tttaagatta acttgtctta aagagatgta gtgcagcatt tgtttatggc ctggaaataa
      481 attaatttag agataaagtc tgtagcaagt acacccagct ttcttgtaca aagtggttga
      541 tctagagggc ccgcagtggg gtcagcttct accggggcgg cgtcccgggg tcttggaact
      601 gcatgcagac tgcctgcttg ggaaacatac ttctttatat gcccatatgg acctgctaag
      661 ctatggaatg taaagaagta tgtatctcag gccgggacct ctctcgccgc actgaggggc
      721 actccacacc acgggggccg ccgcggccga cagccagcca ggcggctgtg gtgggggtga
      781 ggctggggcg gcaggctgcc tgggcgttgg tggacggtcg tgtccccgcc agcattgttc
      841 tgtcactttg cctgtgtcag caggtggaag tgctcaccag acattcgaag gtaagcctat
      901 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttaaac
//
