LOCUS       sequence-67914-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter cel-mir-50
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(377..401)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             415..456
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aaaaagcagg ctcatatggc agattgtgca ggagaacacg gtgagctatt ttctttatta
       61 gaaacattta taaactagga ctctcccaat tcctcttcga ttctattttt actaattctg
      121 aattccataa ttctatattc tgtattcctg cccgccggcc gctgatatgt ctggtattct
      181 tgggtttgaa cttccagcgt tgaacccgca tattagacgt atcgacggcc ggcggggcag
      241 gtaatggctg gtaagtttgg agagaccggg ggagtcccag agatgcatcg accaaaagcc
      301 gaaattactg agcttaatga aaactcttca cgttttcagc aagaaatcgt cgaaacagaa
      361 ttcgacgaca aagcttaccc agctttcttg tacaaagtgg ttcgatggtt cgaaggtaag
      421 cctatcccta accctctcct cggtctcgat tctacgcgtt ctagagcagt ggggtcagct
      481 tctaccgggg cggcgtcccg gggtcttgga actgcatgca gactgcctgc ttgggaaaca
      541 tacttcttta tatgcccata tggacctgct aagctatgga atgtaaagaa gtatgtatct
      601 caggccggga cctctctcgc cgcactgagg ggcactccac accacggggg ccgccgcggc
      661 cga
//
