LOCUS       sequence-67915-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK162_mCherry-4.1G-hardened
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             290..310
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             323..367
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagtccgga ggggatcggt ggagctccac cggtggcggc cgctctagag agaatcttta
      301 ttttcagggc gccgccaaat tcaaagaaac cgctgctgct aaattcgaac gccagcacat
      361 ggacagcgga ggtggaggta ctagtctcga gatgactact gaagtaggct ctgtgtctga
      421 agtgaagaag gactctagcc agttaggaac agatgcaacc aaggaaaaac ctaaagaagt
      481 agcagaaaat cagcagaatc agtcttccga tccagaggag gaaaaaggtt cccagccacc
      541 tcctgcagct gaaagccaaa gtagtctacg ccgccagaag agagagaagg aaacatcgga
      601 gagcaggggt atttctcggt tcataccgcc atggcttaag aagcaaaagt catatacctt
      661 agtagtggcc aaagatggag gagataaaaa agagcctacc caagctgttg ttgaagaaca
      721 ggtcttagat aaagaggaac cccttccaga agaacagaga caggctaagg gtgatgctga
      781 agaaatggct cagaagaaac aagagattaa agttgaagtc aaggaag
//
