LOCUS       sequence-67918-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Okumura F, Zou W, Zhang DE
  TITLE     ISG15 modification of the eIF4E cognate 4EHP enhances cap 
            structure-binding activity of 4EHP.
  JOURNAL   Genes Dev. 2007 Feb 1. 21(3):255-60.
  PUBMED    17289916
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2007 Feb 1. 21(3):255-60."
COMMENT     Sequence Label: pHA-eIF4E
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(800..818)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(801..818)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(838..855)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggag accatgtacc catacgacgt cccagactac gctggaagct tggtaccgat
      121 ggcgactgtc gaaccggaaa ccacccctac tcctaatccc ccgactacag aagaggagaa
      181 aacggaatct aatcaggagg ttgctaaccc agaacactat attaaacatc ccctacagaa
      241 cagatgggca ctctggtttt ttaaaaatga taaaagcaaa acttggcaag caaacctgcg
      301 gctgatctcc aagtttgata ctgttgaaga cttttgggct ctgtacaacc atatccagtt
      361 gtctagtaat ttaatgcctg gctgtgacta ctcacttttt aaggatggta ttgagcctat
      421 gtgggaagat gagaaaaaca aacggggagg acgatggcta attacattga acaaacagca
      481 gagacgaagt gacctcgatc gcttttggct agagacactt ctgtgcctta ttggagaatc
      541 ttttgatgac tacagtgatg atgtatgtgg cgctgttgtt aatgttagag ctaaaggtga
      601 taagatagca atatggacta ctgaatgtga aaacagagaa gctgttacac atatagggag
      661 ggtatacaag gaaaggttag gacttcctcc aaagatagtg attggttatc agtcccacgc
      721 agacacagct actaagagcg gctccaccac taaaaatagg tttgttgttt aactcgagca
      781 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
      841 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccc
//
