LOCUS       sequence-67928-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK165_Mem-mCherry-4.1G-CTD
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..811
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(249..267)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     517..536
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc ggccaccatg ctgtgctgta tgagaagaac
       61 caaacaggtt gaaaagaatg atgacgacca aaagattggc ggcatggtga gcaagggcga
      121 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      241 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      301 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      361 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      421 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      481 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      541 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      601 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      661 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      721 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      781 ctccaccggc ggcatggacg agctgtacaa gtccggaggg gatcggtgga gctccaccgg
      841 tggcggccgc t
//
