LOCUS       sequence-67929-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK165_Mem-mCherry-4.1G-CTD
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(541..585)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(598..618)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgcataacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
      121 gttatctaga tccggtggat cccgggcccg cggttaatct tccccttcct cagccaactc
      181 tgtttctttg tgtaccacca ctcttgtgac cgacatgtca gggtgctgct ctctggcttc
      241 cctgatcgcc tgagccagtg cctggtcatg atcaatatct ccatctcctg tgatcacaat
      301 gcgtttctca attcttgttt cagaaattcc accttttaca gtcttggtga tgtgtgtggt
      361 tgtcgttgtt gacacggact cagatgtgat ggtttgtgcg gtcagtaacg tgcccgaatc
      421 accaccagcc ccgccatcaa tctgtggaga ctcatatgtg atggttttgg tctcagtttg
      481 gacaatgggg acttccttgg tggagatttc tgtcctctcg agactagtac ctccacctcc
      541 gctgtccatg tgctggcgtt cgaatttagc agcagcggtt tctttgaatt tggcggcgcc
      601 ctgaaaataa agattctctc tagagcggcc gccaccggtg gagctccacc gatcccctcc
      661 ggacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      721 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tga
//
