LOCUS       sequence-67935-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of S25N Rab1a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, 
            Hebert TE
  TITLE     Seven transmembrane receptor core signaling complexes are assembled 
            prior to plasma membrane trafficking.
  JOURNAL   J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.
  PUBMED    16959776
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-10"; volume: "281(45)"; pages: "34561-73. 
            Epub 2006 Sep 7"
COMMENT     Sequence Label: PCMV-intron myc Rab1a S25N
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          169..301
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     284..303
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        346..364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             379..408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca
       61 gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagtg
      121 actctcttaa ggtagccttg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      181 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      241 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      301 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
      361 taggctagcc tcgacatgga acagaaactc atctctgaag aggatctgtc tagaggatcc
      421 accatgtcca gcatgaatcc cgaatatgat tatttattca agttacttct gattggcgac
      481 tcaggggttg gaaagaattg ccttcttctt aggtttgcag atgatacata tacagaaagc
      541 tacatcagca caattggtgt ggatttcaaa ataagaacta tagagttaga cgggaaaaca
      601 atcaagcttc aaatatggga cacagcaggc caggaaagat ttcgaacaat cacctccagt
      661 tattacagag gagcccatgg catcatagtt gtgtatgatg tgacagatca ggagtccttc
      721 aataatgtta aacagtggct gcaggaaata gatcgttatg ccagtgaaaa tgtcaacaaa
      781 ttgttggtag ggaacaaatg tgatctgacc acaaagaaag tagtagacta cacaacagcg
      841 aaggaatttg ctgattccct tggaattccg tttttggaaa ccagtgctaa gaatgcaacg
      901 aatgtagaac agtctttcat gacgatggca gctgagatta a
//
