LOCUS       sequence-67936-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pENTR-L4-pTF-L1R
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..137
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    164..182
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    205..223
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    369..387
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    450..573
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(787..806)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(865..884)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtcttaagct cgggcccgag ttaacgctac catggagctc caaataatga ttttattttg
       61 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttttta taatgccaac
      121 tttgtataga aaagttgggt acaactccca ctagacatat cctactctat cattgataga
      181 gtgacccttc catagacata tcctactcta tcattgatag agtgaccctt ccactaggtt
      241 cacttttctc tatcattgat agggagtggt aaacaattga tggacaaaat ctatcaatga
      301 tagagagtgc atgttactag tattgttctt ctctatcatt gatagtgact ttgcctaatc
      361 acttagcaac tctatcattg atagagttct agtattagca aggcctttcc tctatataag
      421 gaagttcatt tcatttggag aagatccccc aagtttgtac aaaaaagttg aacgagaaac
      481 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      541 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga
      601 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
      661 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      721 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      781 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
      841 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
      901 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
      961 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1021 aatttatgcc tcttccgacc atcaagcatt tta
//
