LOCUS       sequence-67938-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of WT Rab8a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, 
            Hebert TE
  TITLE     Seven transmembrane receptor core signaling complexes are assembled 
            prior to plasma membrane trafficking.
  JOURNAL   J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.
  PUBMED    16959776
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-10"; volume: "281(45)"; pages: "34561-73. 
            Epub 2006 Sep 7"
COMMENT     Sequence Label: Rab8WT(Flag) in pcDNA3.1neo
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(812..829)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga
      121 tccaccatgg actacaagga cgatgatgac aaggcgaaga cctacgatta cctgttcaag
      181 ctgctgctga tcggggactc gggggtgggg aagacctgtg tcctgttccg cttctccgag
      241 gacgccttca actccacttt tatctccacc ataggaattg actttaaaat taggaccata
      301 gagctcgatg gcaagagaat taaactgcag atatgggaca cagccggtca ggaacggttt
      361 cggacgatca caacggccta ctacaggggt gcaatgggca tcatgctggt ctacgacatc
      421 accaacgaga agtccttcga caacatccgg aactggattc gcaacattga ggagcacgcc
      481 tctgcagacg tcgaaaagat gatactcggg aacaagtgtg atgtgaatga caagagacaa
      541 gtttccaagg aacggggaga aaagctggcc ctcgactatg gaatcaagtt catggagacc
      601 agcgcgaagg ccaacatcaa tgtggaaaat gcatttttca ctctcgccag agatatcaaa
      661 gcaaaaatgg acaaaaaatt ggaaggcaac agcccccagg ggagcaacca gggagtcaaa
      721 atcacaccgg accagcagaa gaggagcagc tttttccgat gtgttcttct gtgactcgag
      781 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      841 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      901 ctttcctata aatgaggaat t
//
