LOCUS       sequence-67939-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCLX-pTF-R1-DEST-R2-EBR65
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    369..387
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    533..551
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    574..592
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgattcg actctagagg
      301 atccccgggg atcttctcca aatgaaatga acttccttat atagaggaaa ggccttgcta
      361 atactagaac tctatcaatg atagagttgc taagtgatta ggcaaagtca ctatcaatga
      421 tagagaagaa caatactagt aacatgcact ctctatcatt gatagatttt gtccatcaat
      481 tgtttaccac tccctatcaa tgatagagaa aagtgaacct agtggaaggg tcactctatc
      541 aatgatagag taggatatgt ctatggaagg gtcactctat caatgataga gtaggatatg
      601 tctagtggga gttgtaccga accgataccg tcgagatccg ttcactaatc gaatggatct
      661 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
      721 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
      781 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
      841 ttttatatac cacagccaat ttgttatgtt a
//
