LOCUS       sequence-67942-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCLX-pTF-R1-DEST-R2-EBR65
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     294..310
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     295..311
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    326..443
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        500..711
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggtgat tcgacctgca ggcatgcaag ctcgggcccc ccctcgaggt
      301 cgacggtatc gataagcttg ataactttta aaagaaaagg ggggattggg gggtacagtg
      361 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
      421 aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgagaag cttgatcgat
      481 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
      541 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      601 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      661 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtgtcgtga
      721 cgcggatcca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
      781 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
      841 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
      901 gaactcgtgg tgctgg
//
