LOCUS       sequence-67944-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of S20N Rab2a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, 
            Hebert TE
  TITLE     Seven transmembrane receptor core signaling complexes are assembled 
            prior to plasma membrane trafficking.
  JOURNAL   J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.
  PUBMED    16959776
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-10"; volume: "281(45)"; pages: "34561-73. 
            Epub 2006 Sep 7"
COMMENT     Sequence Label: PCMV-intron myc Rab2 S20N
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          170..302
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     285..304
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        347..365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             380..409
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
       61 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      121 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      181 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      241 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
      301 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
      361 ataggctagc ctcgacatgg aacagaaact catctctgaa gaggatctgt ctagaggatc
      421 caccatggcg tacgcctatc tcttcaagta catcataatc ggcgacacag gtgttggtaa
      481 aaactgctta ttgctacagt ttacagacaa gaggtttcag ccagtgcatg accttactat
      541 tggtgtagag ttcggtgctc gaatgataac tattgatggg aaacagataa aacttcagat
      601 atgggatacg gcagggcaag aatcctttcg ttccatcaca aggtcgtatt acagaggtgc
      661 agcaggagct ttactagttt acgatattac acggagagat acattcaacc acttgacaac
      721 ctggttagaa gatgcccgcc agcattccaa ttccaacatg gtcattatgc ttattggaaa
      781 taaaagtgat ttagaatcta gaagagaagt aaaaaaagaa gaaggtgaag cttttgcacg
      841 agaacatgga
//
