LOCUS       sequence-67952-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter cel-mir-230
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(336..360)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             374..415
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aaaaagcagg ctcatatgtc cttctttagc gatcaaccat aaaaccccgc ctccgttttg
       61 aaacatgaaa aaaagtataa aaatagaata ccacaacgac acttgttaaa atattattcc
      121 tcatatctag cttctgcttg tatttttttg tctgatgttg atgtaatgcc gtcacttggt
      181 cggcgattta atattatcag aacataggaa attgttagta ttagttgtgc gaccaggaga
      241 cggtattcgc atatttctta aatattcatt ttcataatcg tacaaggtac gtttcgagta
      301 atgtctgcaa ggttcttgcc cacaatgaca agcttaccca gctttcttgt acaaagtggt
      361 tcgatggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgttc
      421 tagagcagtg gggtcagctt ctaccggggc ggcgtcccgg ggtcttggaa ctgcatgcag
      481 actgcctgct tgggaaacat acttctttat atgcccatat ggacctgcta agctatggaa
      541 tgtaaagaag tatgtatctc aggccgggac ctctctcgcc gcactgaggg gcactccaca
      601 ccacgggggc cgccgcggcc gacagccagc caggcggctg tggtgggggt gaggctgggg
      661 cggcaggctg cctgggcgtt
//
