LOCUS       sequence-67954-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pMT mir-1-1 reporter cel-mir-240
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(326..350)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             364..405
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aaaaagcagg ctcatatgtc atgagctccg cctttactat tctatccacc acctacatat
       61 attacagttt catttgcaca cggcggcttg tcattttcct gtctattgct atttcgttgt
      121 tagaaacttt ttcaaatcga ggattttgag actagaatgc ttgaattgta ctagcatact
      181 ggcccccaaa tcttcgctta gaaatacagt tcacaattat tttaatggtg agtaaagtaa
      241 gtaaagggat agttcccgat cgaatatcag ttggggtatt tacaaaaaga caaaattgta
      301 atgccctgaa tgatgttcaa agcttaccca gctttcttgt acaaagtggt tcgatggttc
      361 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgttc tagagcagtg
      421 gggtcagctt ctaccggggc ggcgtcccgg ggtcttggaa ctgcatgcag actgcctgct
      481 tgggaaacat acttctttat atgcccatat ggacctgcta agctatggaa tgtaaagaag
      541 tatgtatctc aggccgggac ctctctcgcc gcactgaggg gcactccaca ccacgggggc
      601 cgccgcggcc gacagccagc caggcggctg tggtgggggt ga
//
