LOCUS       sequence-67955-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-124
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(509..533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttc attgattcac
      121 atccattttg agaaggatgg ttgctactgc tgcttgatct caaggactgt gtgctcccac
      181 ttttactcaa agtattttat ttcttcttca agtgctctct tctccatttt tgaatttcct
      241 tcgcaattga tacaccatgt cccacttgtc atctggcatg caccctagtg actttagtgg
      301 acatctaagt cttccaacta aggcacgcgg tgaatgccac gtggccatga tggggctccc
      361 attgaatttg aaaggttggt tgatctatct atctatctct tcatagttat tcatccatat
      421 cggtgtcaca gaatgaaagc gagagagctg actaattcat gtacatttga tgctctttcc
      481 ctcccatttt gcgtccattc attaggatac ccagctttct tgtacaaagt ggttgatcta
      541 gagggcccgc agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat
      601 gcagactgcc tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat
      661 ggaatgtaaa gaagtatgta tctcaggccg ggacctctct cgccgcactg aggggcactc
      721 cacaccacgg ggg
//
