LOCUS       sequence-67956-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-235
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(506..530)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctgg accttcaatt
      121 ggtaccaatc gaaactcgtt ttcttctaag tgtatgtatg tgtgtccttt cttcagatga
      181 caccacctgt ggatggttct taattttcta tctattcctt attcttccaa gtgctatcta
      241 ttcgtttgct gaaaatcgtc cgaagatatc aggatcaggc cttggctgat tgcaaaattg
      301 ttcaccgtga aaattaaata ttgcactctc cccggcctga tctgagagta aggcgaagct
      361 gaattgactt taattcgaca ggtttgtttt tgcttcaatt gtttaaaatt tttaaatggg
      421 ttttagtttt agcaggtttc tttatatact ctgtggaatt ttttaaaagt atctgttgtg
      481 ttataatgtg ctaatagaaa acaaaaccca gctttcttgt acaaagtggt tgatctagag
      541 ggcccgcagt ggggtcagct tctaccgggg cggcgtcccg gggtcttgga actgcatgca
      601 gactgcctgc ttgggaaaca tacttcttta tatgcccata tggacctgct aagctatgga
      661 atgtaaagaa gtatgtatct caggcc
//
