LOCUS       sequence-67957-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter dme-let-7
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(487..511)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttc aaaagcattt
      121 ttaatatgat ttctccgtat tatttttctt cttgtttgcc atcatcgttt caaccaaaaa
      181 ccgaaccaat gatatccaga agatccttta aataccaaac cacctagcaa aaaggactac
      241 accaaggacc tttttctctc tggcaaattg aggtagtagg ttgtatagta gtaattacac
      301 atcatactat acaatgtgct agctttcttt gcttgactac aagccgcatt tgataaaaga
      361 atcccaatcg aactgcacca cttaataaac ccaatcccca gccatacaaa agttggtggt
      421 gcaacaaatt ttgattgtgg acaacaaaga aagtgttctg agccaaacta ttgttaaata
      481 tcattaaccc agctttcttg tacaaagtgg ttgatctaga gggcccgcag tggggtcagc
      541 ttctaccggg gcggcgtccc ggggtcttgg aactgcatgc agactgcctg cttgggaaac
      601 atacttcttt atatgcccat atggacctgc taagctatgg aatgtaaaga agtatgtatc
      661 tcagg
//
