LOCUS       sequence-67960-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter dme-mir-2a-1
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(485..509)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctta attgaacaca
      121 aaaagtataa aaacggtaac aagtgtctga ttttgtttat atcattacat gagcatgaaa
      181 accaaaattg aaaattctga actcccgcag tcaagtatcc cgaaacctca atcaataaaa
      241 ctatattggc gcattcatgc tgggctctca aagtggttgt gaaatgcatt tccgctttgc
      301 gcggcatatc acagccagct ttgatgagct tagcgtaatg tatcgtacat caaatcataa
      361 taatacctcc cgccacataa ttcacacgcc catcataaat aaaacacact atgtccgcct
      421 caaatctgta taatatttgt agtgaaatgc aatcggaaaa taaacgcggc taacagagat
      481 ctgaacccag ctttcttgta caaagtggtt gatctagagg gcccgcagtg gggtcagctt
      541 ctaccggggc ggcgtcccgg ggtcttggaa ctgcatgcag actgcctgct tgggaaacat
      601 acttctttat atgcccatat ggacctgcta agctatggaa tgtaaagaag tatgtatctc
      661 aggccgggac ctctctcgcc gcactgaggg gcactccaca ccacgggggc cgccgcggcc
      721 gacagccagc caggcggctg tggtgggggt ga
//
