LOCUS       sequence-67961-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-46
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(510..534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctac ttctcctgca
      121 gaccgtgcgc gggagggggt cttcagccat tcattcaatg tctcaccttc caactacttt
      181 tcttttctca cctattccct atttaattgt attttcctat gtattttgaa cgaaaagttg
      241 aaggtaaggg tccaaactgc tgaggtgaag ctgaagagag ccgtctattg acagttcaag
      301 accacgagtc gttgtgtgct gtcatggagt cgctctcttc agatgatccg gtcaatggta
      361 ttttgtttta atataatatt ttgcaaaaaa aaataatttg aatgaatttt atttcgagga
      421 gtttttaatc gaaaagttca cattaaaata ttgaaagttc cgaaactcta aatttgaaaa
      481 caattcttaa aatcttcgct tattccaaaa cccagctttc ttgtacaaag tggttgatct
      541 agagggcccg cagtggggtc agcttctacc ggggcggcgt cccggggtct tggaactgca
      601 tgcagactgc ctgcttggga aacatacttc tttatatgcc catatggacc tgctaagcta
      661 tggaatgtaa agaagtatgt atctcaggcc gggacctctc tcgccgcact gaggggcact
      721 ccacaccacg ggggccgccg cggccgacag ccagccaggc ggctgtggtg ggggtga
//
