LOCUS       sequence-67962-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-59
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(509..533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttc atccacctgt
      121 ttctcagtgt ctcttagtta ttacactatt acacatggcg ccaataaaag gacctcttag
      181 aatgggaagg gccgttcact tgttcgtttc attctacatc actttactgc tttctttatt
      241 tattcttatg atattttgag ttgatctaga tatgacatcg tcctgaaaac gaaacggaac
      301 aaaagttcaa gatattgatt tcgaatcgtt tatcaggatg atgtgattaa aatcaactcg
      361 gacggtaagc gagagttttc aattattttt aacttttttt tggtaagatt taactactat
      421 tgagttactt tagatatatg taagaagttt agatatatta aaggagctga tttttttaaa
      481 aaattattta tggtcaggaa tttcttgaac ccagctttct tgtacaaagt ggttgatcta
      541 gagggcccgc agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat
      601 gcagactgcc tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat
      661 ggaatgtaaa gaagtatgta tctcaggccg ggacctctct cgccgcactg aggggcactc
      721 cacaccacgg gggccgccgc ggccgacagc cagccaggcg gctgtggtgg gggtga
//
