LOCUS       sequence-67963-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-60
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(507..531)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             884..925
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttg gtgataggca
      121 accatctttt ctgtgtctca actatctacc attccataag cccacctact tttgtgtcac
      181 tataaaagag aattgtggtg taggtgataa cagtgctctt tgtattatta tctacaatta
      241 cttcgtattc atattctact cgaaaaccgc ttgttcttga actggaagag tgccataaaa
      301 tcatgacaaa gtacgtgata ttatgcacat tttctagttc aagacttgag aaatcggtta
      361 actgtttttg aaacaacttc tgatttttgt tgacggtagt ttttgctatc atgagttccg
      421 ggtaaatatc atatactgta agacatttgt tagaaatcac aatgtttctt tgattgatta
      481 tttcaatttg cttatgggaa actcttaccc agctttcttg tacaaagtgg ttgatctaga
      541 gggcccgcag tggggtcagc ttctaccggg gcggcgtccc ggggtcttgg aactgcatgc
      601 agactgcctg cttgggaaac atacttcttt atatgcccat atggacctgc taagctatgg
      661 aatgtaaaga agtatgtatc tcaggccggg acctctctcg ccgcactgag gggcactcca
      721 caccacgggg gccgccgcgg ccgacagcca gccaggcggc tgtggtgggg gtgaggctgg
      781 ggcggcaggc tgcctgggcg ttggtggacg gtcgtgtccc cgccagcatt gttctgtcac
      841 tttgcctgtg tcagcaggtg gaagtgctca ccagacattc gaaggtaagc ctatccctaa
      901 ccctctcctc ggtctcgatt ctacgcgtac cggt
//
