LOCUS       sequence-67968-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Giry-Laterriere M, Verhoeyen E, Salmon P
  TITLE     Lentiviral vectors.
  JOURNAL   Methods Mol Biol. 2011;737:183-209. doi: 
            10.1007/978-1-61779-095-9_8.
  PUBMED    21590398
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2011"; volume: "737:183-209. doi"; pages: " 
            10.1007/978-1-61779-095-9_8"
COMMENT     Sequence Label: pCLX-R4-DEST-R2
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(342..362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(760..771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg agcagatctg ctcgaccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agctttcttg tacaaagtgg tgataatcga attccgataa tcaacctctg
      301 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      361 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
      421 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
      481 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
      541 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
      601 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
      661 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
      721 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
      781 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttc
//
