LOCUS       sequence-67974-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-hairy
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..149
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          196..262
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             298..318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             319..345
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
       61 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
      121 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
      181 gaatagatct aaaaggtagg ttcaaccact gatgcctagg cacaccgaaa cgactaaccc
      241 taattcttat cctttacttc aggcggccgc ggctcgagca aaatggcttc ctcccctcca
      301 aagaaaaaga gaaaggttag ttggaaggac gcaagtggtt ggtctagaat gcatgcggat
      361 cccattcgtc cgcgcaggcc aagtcctgcc cgcgagcttc tgcccggacc ccaaccggat
      421 agggttcagc cgactgcaga tcgtggggtg tctgcgcctg ctggcagccc tctggatggc
      481 ttgcccgctc ggcggacggt gtcccggacc cggctgccat ctccccctgc gccctcacct
      541 gcgttctcgg cgggcagctt cagcgatctg ctccgtccgt tcgatccgtc gcttcttgat
      601 acatcgcttc ttgattcgat gcctgccgtc ggcacgccgc atacagcggc tgccccagca
      661 gagtgggatg aggcgcaatc ggctctgcgt gcagccgatg acccgccacc caccgtgcgt
      721 gtcgctgtca ctgccgcgcg gccgccgcgc gccaagccgg ccccgcgacg gcgtgctgcg
      781 caaccctccg acgcttcgcc ggccgcgcag gtggatctac gcacgctcgg ctacagtcag
      841 cagcagcaag agaagatcaa accg
//
