LOCUS       sequence-67975-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-hairy
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..54
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     46..63
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     47..63
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(139..159)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(139..157)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(178..194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(178..194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(191..213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(226..232,233..250,251..256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    271..292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
       61 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
      121 gtcctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
      181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      301 gcgctcactg cccgctttcc accggtcgtc tccaacgacc acctcgtcgc cttggcctgc
      361 ctcggcggac gtcctgccat ggatgcagtg aaaaagggat tgccgcacgc gccggaattg
      421 atcagaagag tcaatcgccg tattggcgaa cgcacgtccc atcgcgttgc cgactacgcg
      481 caagtggttc gcgtgctgga gtttttccag tgccactccc acccagcgta cgcatttgat
      541 gaggccatga cgcagttcgg gatgagcagg aacgggttgg tacagctctt tcgcagagtg
      601 ggcgtcaccg aactcgaagc ccgcggtgga acgctccccc cagcctcgca gcgttgggac
      661 cgtatcctcc aggcatcagg gatgaaaagg gccaaaccgt cccctacttc agctcaaaca
      721 ccggatcagg cgtctttgca tgcattcgcc gattcgctgg agcgtgacct tgatgcgccc
      781 agcccaatgc acgagggaga tcagacgcgg gcaagcagcc gtaaacggtc ccgatcggat
      841 cgtgctgtca ccggcccctc cgcacag
//
