LOCUS       sequence-67981-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-Kr
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    197..291
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    325..419
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    453..547
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    581..675
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     885..906
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
       61 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
      121 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag ggccgcaagc
      181 ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      241 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc
      301 tagccctagg gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
      361 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      421 ggagactcta gcgctagcgc atgcctgcag gtcggagtac tgtcctccga gcggagtact
      481 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
      541 ctccgagcgg agactctagc actagtgcat gcctgcaggt cggagtactg tcctccgagc
      601 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
      661 gtactgtcct ccgagcggag actctagcga cgtcgagcgc cggagtataa atagaggcgc
      721 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
      781 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt
      841 aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact actgaaatct
      901 gccaagaagt aattattgat a
//
