LOCUS       sequence-67984-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-Kr
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..148
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          195..261
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             297..317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             318..344
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
       61 aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa
      121 tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa gagaactctg
      181 aatagatcta aaaggtaggt tcaaccactg atgcctaggc acaccgaaac gactaaccct
      241 aattcttatc ctttacttca ggcggccgcg gctcgagcaa aatggcttcc tcccctccaa
      301 agaaaaagag aaaggttagt tggaaggacg caagtggttg gtctagaatg catgcggatc
      361 ccattcgtcc gcgcaggcca agtcctgccc gcgagcttct gcccggaccc caaccggata
      421 gggttcagcc gactgcagat cgtggggtgt ctgcgcctgc tggcagccct ctggatggct
      481 tgcccgctcg gcggacggtg tcccggaccc ggctgccatc tccccctgcg ccctcacctg
      541 cgttctcggc gggcagcttc agcgatctgc tccgtccgtt cgatccgtcg cttcttgata
      601 catcgcttct tgattcgatg cctgccgtcg gcacgccgca tacagcggct gccccagcag
      661 agtgggatga ggcgcaatcg gctctgcgtg cagccgatga cccgccaccc accgtgcgtg
      721 tcgctgtcac tgccgcgcgg ccgccgcgcg ccaagccggc cccgcgacgg cgtgctgcgc
      781 aaccctccga cgcttcgccg gccgcgcagg tggatctacg cacgctcggc tacagtcagc
      841 agcagcaaga gaagatcaaa
//
