LOCUS       sequence-67989-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-Kr
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..31
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     23..40
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     24..40
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(116..136)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(116..134)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(155..171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(155..171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(168..190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(203..209,210..227,228..233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    248..269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
       61 actatagggc gaattgggta ccgggccccc cctcgaggtc ctccagcttt tgttcccttt
      121 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccacc
      301 ggtcgtctcc aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga
      361 tgcagtgaaa aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat
      421 tggcgaacgc acgtcccatc gcgttgccga ctacgcgcaa gtggttcgcg tgctggagtt
      481 tttccagtgc cactcccacc cagcgtacgc atttgatgag gccatgacgc agttcgggat
      541 gagcaggaac gggttggtac agctctttcg cagagtgggc gtcaccgaac tcgaagcccg
      601 cggtggaacg ctccccccag cctcgcagcg ttgggaccgt atcctccagg catcagggat
      661 gaaaagggcc aaaccgtccc ctacttcagc tcaaacaccg gatcaggcgt ctttgcatgc
      721 attcgccgat tcgctggagc gtgaccttga tgcgcccagc ccaatgcacg agggagatca
      781 gacgcgggca agcagccgta aacggtcccg atcggatcgt gctgtcaccg gcccctccgc
      841 acagca
//
