LOCUS       sequence-68000-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-VP64
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     135..156
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          203..269
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             305..325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             326..352
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
       61 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
      121 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
      181 gaactctgaa tagatctaaa aggtaggttc aaccactgat gcctaggcac accgaaacga
      241 ctaaccctaa ttcttatcct ttacttcagg cggccgcggc tcgagcaaaa tggcttcctc
      301 ccctccaaag aaaaagagaa aggttagttg gaaggacgca agtggttggt ctagaatgca
      361 tgcggatccc attcgtccgc gcaggccaag tcctgcccgc gagcttctgc ccggacccca
      421 accggatagg gttcagccga ctgcagatcg tggggtgtct gcgcctgctg gcagccctct
      481 ggatggcttg cccgctcggc ggacggtgtc ccggacccgg ctgccatctc cccctgcgcc
      541 ctcacctgcg ttctcggcgg gcagcttcag cgatctgctc cgtccgttcg atccgtcgct
      601 tcttgataca tcgcttcttg attcgatgcc tgccgtcggc acgccgcata cagcggctgc
      661 cccagcagag tgggatgagg cgcaatcggc tctgcgtgca gccgatgacc cgccacccac
      721 cgtgcgtgtc gctgtcactg ccgcgcggcc gccgcgcgcc aagccggccc cgcgacggcg
      781 tgctgcgcaa ccctccgacg cttcgccggc cgcg
//
