LOCUS       sequence-68004-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-GFP
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..809)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(142..162)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     757..785
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac
       61 accacagaag taaggttcct tcacaaagat cctctagact atttgtatag ttcatccatg
      121 ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtggtc tctcttttcg
      181 ttgggatctt tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg taaaaggaca
      241 gggccatcgc caattggagt attttgttga taatggtctg ctagttgaac gcttccatct
      301 tcaatgttgt gtctaatttt gaagttaact ttgattccat tcttttgttt gtctgccatg
      361 atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat gtttccatct
      421 tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc ttcaaacttg
      481 acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct ttcctggaca
      541 taaccttcgg gcatggcact cttgaaaaag tcatgctgtt tcatatgatc tgggtatctt
      601 gaaaagcatt gaacaccata agttaaagta gtgacaagtg ttggccatgg aacaggtagt
      661 tttccagtag tgcaaataaa tttaagggta agttttccgt atgttgcatc accttcaccc
      721 tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac aagaattggg
      781 acaactccag tgaaaagttc ttctcctttc tgggatctag atatcggatc cgggaggccg
      841 ccccagatcc tggtacgcgg gcgttttacc ctcacgagga agac
//
