LOCUS       sequence-68011-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-GFP
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     132..153
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          200..266
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             302..322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             323..349
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
       61 taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa
      121 gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa
      181 ctctgaatag atctaaaagg taggttcaac cactgatgcc taggcacacc gaaacgacta
      241 accctaattc ttatccttta cttcaggcgg ccgcggctcg agcaaaatgg cttcctcccc
      301 tccaaagaaa aagagaaagg ttagttggaa ggacgcaagt ggttggtcta gaatgcatgc
      361 ggatcccatt cgtccgcgca ggccaagtcc tgcccgcgag cttctgcccg gaccccaacc
      421 ggatagggtt cagccgactg cagatcgtgg ggtgtctgcg cctgctggca gccctctgga
      481 tggcttgccc gctcggcgga cggtgtcccg gacccggctg ccatctcccc ctgcgccctc
      541 acctgcgttc tcggcgggca gcttcagcga tctgctccgt ccgttcgatc cgtcgcttct
      601 tgatacatcg cttcttgatt cgatgcctgc cgtcggcacg ccgcatacag cggctgcccc
      661 agcagagtgg gatgaggcgc aatcggctct gcgtgcagcc gatgacccgc cacccaccgt
      721 gcgtgtcgct gtcactgccg cgcggccgcc gcgcgccaag ccggccccgc gacggcgtgc
      781 tgcgcaaccc tccgacgctt cgccggccgc gcaggtggat ctacgcacgc tcggctacag
      841 tcagcagcag caagagaaga tcaaaccga
//
