LOCUS       sequence-68016-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, 
            Ramirez-Alvarado M
  TITLE     Tyrosine residues mediate fibril formation in a dynamic light chain 
            dimer interface.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 
            10.1074/jbc.M112.362921. Epub 2012 Jun 27.
  PUBMED    22740699
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362921"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27997-8006"
COMMENT     Sequence Label: pET12a-AL-09 Y32F Y96F
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(575..593)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      589..636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatgcgggcg aaactcctag gaatagtcct gacaacccct atcgcgatca
      181 gctcttttgc gtcgacggac atccagatga cccagtctcc atcctccctg tctgcatctg
      241 taggcgacag agtcaccatc acttgccagg cgagtcagga cattaacaac tttttaattt
      301 ggtatcagca gaagccaggg caagccccta agctcctgat ctacgatgca tccactttgg
      361 aaacaggggt cccatcaagg ttcagtggaa gtggatctgg gacagaattc actttcacca
      421 tcagcagcct gcagcctgag gatcttgcaa catatcactg tcaacagtat gataatctcc
      481 cgttcacttt tggccagggg accaagctgg agatcaaacg ataaggatcc ggctgctaac
      541 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      601 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      661 tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
      721 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
      781 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
      841 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
      901 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tc
//
