LOCUS       sequence-68021-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, 
            Ramirez-Alvarado M
  TITLE     Tyrosine residues mediate fibril formation in a dynamic light chain 
            dimer interface.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 
            10.1074/jbc.M112.362921. Epub 2012 Jun 27.
  PUBMED    22740699
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362921"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27997-8006"
COMMENT     Sequence Label: pET12a-AL-09 Y96F
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(576..594)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      590..637
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagaaata attttgttta actttaagaa
      121 ggagatatac atatgcgggc gaaactccta ggaatagtcc tgacaacccc tatcgcgatc
      181 agctcttttg cgtcgacgga catccagatg acccagtctc catcctccct gtctgcatct
      241 gtaggcgaca gagtcaccat cacttgccag gcgagtcagg acattaacaa ctatttaatt
      301 tggtatcagc agaagccagg gcaagcccct aagctcctga tctacgatgc atccactttg
      361 gaaacagggg tcccatcaag gttcagtgga agtggatctg ggacagaatt cactttcacc
      421 atcagcagcc tgcagcctga ggatcttgca acatatcact gtcaacagta tgataatctc
      481 ccgttcactt ttggccaggg gaccaagctg gagatcaaac gataaggatc cggctgctaa
      541 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      601 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      661 atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg
      721 aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc
      781 atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt
      841 cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta
      901 cggcatccag g
//
