LOCUS       sequence-68036-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, 
            Ramirez-Alvarado M
  TITLE     Tyrosine residues mediate fibril formation in a dynamic light chain 
            dimer interface.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 
            10.1074/jbc.M112.362921. Epub 2012 Jun 27.
  PUBMED    22740699
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362921"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27997-8006"
COMMENT     Sequence Label: pET12a-AL-103 Y91F
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(586..604)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      600..647
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcgggcgaa actcctagga atagtcctga caacccctat
      181 cgcgatcagc tcttttgcgt cgacggacat ccagatgacc cagtctccat cctccctgtc
      241 tgcatctgta ggagacagag tcaccatcac ttgccaggcg agtcaggaca ttagtaatta
      301 tttaatttgg tatcaacaga aaccagggaa agcccctaaa ctcctgatct acgatgcatc
      361 caatttggaa acaggggtcc catcaaggtt cagtggaagt ggatctggga cagattttac
      421 tttcaccatc agcagcctgc agcctgaaga tattgcaaca tattactgtc aacagtttca
      481 taatctccct ccgtacactt ttggcccggg gaccaagctg gagatcaaac gataaggatc
      541 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      601 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      661 ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct
      721 ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga
      781 acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg
      841 gcgatgctgt cggaatggac gata
//
