LOCUS       sequence-68037-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, 
            Ramirez-Alvarado M
  TITLE     Tyrosine residues mediate fibril formation in a dynamic light chain 
            dimer interface.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 
            10.1074/jbc.M112.362921. Epub 2012 Jun 27.
  PUBMED    22740699
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362921"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27997-8006"
COMMENT     Sequence Label: pET12a-AL-103 Y96F
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(578..596)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      592..639
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatgcgggcg aaactcctag gaatagtcct gacaacccct atcgcgatca
      181 gctcttttgc gtcgacggac atccagatga cccagtctcc atcctccctg tctgcatctg
      241 taggagacag agtcaccatc acttgccagg cgagtcagga cattagtaat tatttaattt
      301 ggtatcaaca gaaaccaggg aaagccccta aactcctgat ctacgatgca tccaatttgg
      361 aaacaggggt cccatcaagg ttcagtggaa gtggatctgg gacagatttt actttcacca
      421 tcagcagcct gcagcctgaa gatattgcaa catattactg tcaacagtat cataatctcc
      481 ctccgttcac ttttggcccg gggaccaagc tggagatcaa acgataagga tccggctgct
      541 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      601 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      661 ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag
      721 cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc
      781 gcatagaaat tgcatcaacg ca
//
