LOCUS       sequence-68038-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a nuclear-targeted, genetically-encoded sensor for lactate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Barros LF, San Martin A, Sotelo-Hitschfeld T, Lerchundi R, 
            Fernandez-Moncada I, Ruminot I, Gutierrez R, Valdebenito R, Ceballo 
            S, Alegria K, Baeza-Lehnert F, Espinoza D
  TITLE     Small is fast: astrocytic glucose and lactate metabolism at cellular
            resolution.
  JOURNAL   Front Cell Neurosci. 2013;7:27. doi: 10.3389/fncel.2013.00027. Epub 
            2013 Mar 22.
  PUBMED    23526722
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2013"; volume: "7:27. doi"; pages: " 
            10.3389/fncel.2013.00027. Epub 2013 Mar 22"
COMMENT     Sequence Label: NLS-Laconic/pCMV-myc-nuc
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..35)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(48..68)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(72..92)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(96..116)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(129..380)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(171..192)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     520..539
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     779..800
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccattcagat cctcttctga gatgagtttt tgttctgcgg ccgtatctac ctttctcttc
       61 ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt ttttggatct
      121 gcggccgcct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      181 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc
      241 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      301 tggtcggcga gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg
      361 atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc
      421 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      481 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      541 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      601 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc cagggtggtc
      661 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatcagctt cagggtcagc
      721 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
      781 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttg
//
