LOCUS       sequence-68046-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-AL-103 delProIns
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(583..601)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      597..644
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcgggcgaa actcctagga atagtcctga caacccctat
      181 cgcgatcagc tcttttgcgt cgacggacat ccagatgacc cagtctccat cctccctgtc
      241 tgcatctgta ggagacagag tcaccatcac ttgccaggcg agtcaggaca ttagtaatta
      301 tttaatttgg tatcaacaga aaccagggaa agcccctaaa ctcctgatct acgatgcatc
      361 caatttggaa acaggggtcc catcaaggtt cagtggaagt ggatctggga cagattttac
      421 tttcaccatc agcagcctgc agcctgaaga tattgcaaca tattactgtc aacagtatca
      481 taatctccct tacacttttg gcccggggac caagctggag atcaaacgat aaggatccgg
      541 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      601 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      661 tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca
      721 agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg
      781 ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg
      841 atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct
      901 atgcctacag catccagggt gacggtg
//
