LOCUS       sequence-68048-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-DAH
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(584..602)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      598..645
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag
      121 aaggagatat acatatgcgg gcgaaactcc taggaatagt cctgacaacc cctatcgcga
      181 tcagctcttt tgcgtcgacg cagtctgtgt tgacgcagcc gccctcagtg tctgcggccc
      241 caggacagac ggtcaccatc tcctgctctg gaagcagctc caacattggg aataattatg
      301 tatcttggta ccagcacctc ccaggaacag cccccaaatt cctcatttat gacaataata
      361 agcgaccctc agggattcct gaccgattct ctggcttcaa gtctggcacg tcagccaccc
      421 tgggcatcac cggactccag actggggacg aggccgatta ttactgcgga acatgggata
      481 gtagcctgag tgctctggtc ttcggcggag ggaccaagct gaccgtccta taaggatccg
      541 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      661 atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
      721 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
      781 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
      841 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtacc
//
