LOCUS       sequence-68052-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-JOH1
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(575..593)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      589..636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat agggagacca caacggtttc cctctagaaa taattttgtt taactttaag
      121 aaggagatat acatatgcgg gcgaaactcc taggaatagt cctgacaacc cctatcgcga
      181 tcagctcttt tgcgtcgacg gacatccaga tgacccagtc tccatcctca gtgtctgcat
      241 atgttggcga cagagtcacc atcacttgtc gggcgagtca gggcattaat atccgtttag
      301 cctggtttca gcagaagcca gggaaagccc ctgagtctct gatttatggt gcttccactt
      361 tgcaaagtgg tgtcccatca aagttcagcg gcagtggttc tgggacagat ttcactctca
      421 ccatcagcag cctgcggcct gaagattctg caacttatta ctgtcaacaa tataaaagtt
      481 ttccgctcac cttcggccaa gggacgcggc tggagataaa ataaggatcc ggctgctaac
      541 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      601 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      661 tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
      721 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
      781 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
      841 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
      901 agcatccagg gtgacggtgc cgaggatgac gatgagcgca tgtaa
//
