LOCUS       sequence-68066-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of murine CD8mCherry in insects.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     pQUASp plasmids for germline expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQUASp-mCD8mCherry
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..787)
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHS"
     primer_bind     complement(812..828)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..829)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
       61 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatgc
      121 tagcgctgtg gtagcagatg agagtgatga tcaaggacag cagaagggcc acgcagattc
      181 cggccaaggg tgcccagatg taaatatcac aggcgaagtc caatccggtc cccttcactg
      241 agccacgggg ccgacaatct tctggtctct ggggctgaga tgtcccggta gggtgcacag
      301 gtgagggagt tcgcagcact ggcttggtag tagtagagtt cactttctga aggactggca
      361 cgacagaact gaagtacatc accgagttgc tgatgactga gcagaaatag tagccttcgt
      421 tttccttgct gaacttgttc agggtgagaa cgtacttatt attcgtgtcc ctcatggcag
      481 aaaacagttt cgacgaattg agcttctcgt cccacgttat cttgttgtgg gatgaagcca
      541 tatagacaac gaaggtgggc tgggggagtt tggagctgga gttctggaag agccaagagc
      601 atccttgcga aacggacccc aacacttcac ataccaggtc caccttctga ccaagttcgg
      661 cgtccatttt ctttggaaag attcggagtt cgggtgcctg tggcttagct tctccactcc
      721 ccaggataat cgactcaccc agcagcagca ggttcagcga cagaaagcgg gtcaacggtg
      781 aggccatttt ggtaccgcgc atcaagctta tcgataccgt cgacctcgag ccgcggccgc
      841 ggatctgatc cccgggcggg taccaatgaa caggacctaa cgcacagtca cgttattgtt
      901 tacataaatg atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata
      961 gccttctt
//
