LOCUS       sequence-68089-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Rose T, Schoenenberger P, Jezek K, Oertner TG
  TITLE     Developmental refinement of vesicle cycling at schaffer collateral 
            synapses.
  JOURNAL   Neuron. 2013 Mar 20;77(6):1109-21. doi: 
            10.1016/j.neuron.2013.01.021.
  PUBMED    23522046
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.01"; journalName: "Neuron"; date: 
            "2013-03-20- 20"; volume: "77"; issue: "6"; pages: "1109-21"
COMMENT     Sequence Label: pMH4-Syn1-Ratio2XsypHy
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             272..298
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      302..311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      306..315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(441..459)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(491..511)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 gcgcgcacct ccaggcgccc cagaaaagca accagctcct ggcgatgcct acggcgatgc
       61 gggctatggg cagggccccg gaggctatgg gccccaggac tcctacgggc ctcagggtgg
      121 ttatcaaccc gattacgggc agccagccag cggcggtggc ggtggctacg ggcctcaggg
      181 cgactatggg cagcaaggct acggccaaca gggtgcgccc acctccttct ccaatcagat
      241 gtcggatccg aagcttcggt accaagctgc ctacccttac gatgtgcccg attacgccgc
      301 ggccgccatg gtggcctcct ccgaggacgt catcaaagag ttcatgcgct tcaaggtgcg
      361 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      421 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      481 ctgggacatc ctgtcccccc agttccagta cggctccaag gcgtacgtga agcaccccgc
      541 cgacatcccc gactaca
//
