LOCUS       sequence-68091-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A monomeric and improved-activity variant of pea ascorbate 
            peroxidase. Used for electron microscopy and live-cell proteomic 
            mapping. This construct has been replaced by APEX2 (construct 
            49386)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pUC57 APEX
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(791..807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(791..807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(804..826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    815..831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(839..845,846..863,864..869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgagcccaga ttaccaggac gctattgaga aggcaaaaag gaaactgagg ggattcatcg
       61 cagaaaagaa atgcgcccca ctgatcctgc gactggcttt ccacagcgca ggaacatttg
      121 actccaagac caaaacaggc ggaccctttg ggactatcaa gcaccaggct gaactggcac
      181 atggggccaa caatggcctg gacattgctg tgagactgct ggagccaatc aaagaacagt
      241 tccccattgt ctcctacgca gacttctacc agctggccgg cgtggtcgct gtggagatta
      301 ccggcggccc caaggtccct ttccacccag gaagggagga caaacctgaa ccccctccag
      361 aggggagact gccagatgct accaagggca gcgaccatct gcgggacgtg ttcggaaaag
      421 caatggggct gtctgaccag gatatcgtgg ccctgagtgg agggcacacc attggcgccg
      481 ctcataagga acgctcaggc ttcgagggac cctggacaag caaccctctg atcttcgaca
      541 attcctactt tactgaactg ctgaccggcg agaaggatgg actgctgcag ctgccctctg
      601 acaaagccct gctgacagat agcgtgttca ggcctctggt cgaaaagtat gcagccgacg
      661 aggatgtgtt cttcgccgac tatgctgagg cacacctgaa actgtctgaa ctgggattcg
      721 ccgaggcaat tggatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg
      781 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      841 acatacgagc cggaagcata aagtgtaaa
//
