LOCUS       sequence-68096-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     Collier lab plasmids
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-22b PA K397D/D425K
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     50..115
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgaaatacct
       61 gctgccgacc gctgctgctg gtctgctgct cctcgctgcc cagccggcga tggccatgga
      121 tatcggaatt aattcggatc cgatggaagt taaacaggag aaccggttat taaatgaatc
      181 agaatcaagt tcccaggggt tactaggata ctattttagt gatttgaatt ttcaagcacc
      241 catggtggtt acctcttcta ctacagggga tttatctatt cctagttctg agttagaaaa
      301 tattccatcg gaaaaccaat attttcaatc tgctatttgg tcaggattta tcaaagttaa
      361 gaagagtgat gaatatacat ttgctacttc cgctgataat catgtaacaa tgtgggtaga
      421 tgaccaagaa gtgattaata aagcttctaa ttctaacaaa atcagattag aaaaaggaag
      481 attatatcaa ataaaaattc aatatcaacg agaaaatcct actgaaaaag gattggattt
      541 caagttgtac tggaccgatt ctcaaaataa aaaagaagtg atttctagtg ataacttaca
      601 attgccagaa ttaaaacaaa aatcttcgaa ctcaagaaaa aagcgaagta caagtgctgg
      661 acctacggtt ccagaccgtg acaatgatgg aatccctgat tcattagagg tagaaggata
      721 tacggttgat gtcaaaaata aaagaacttt tctttcacca tggatttcta atattcatga
      781 aaagaaagga ttaaccaaat ataaatcatc tcctgaaaaa tggagcacgg cttctgatcc
      841 gtacagtgat ttcgaaaagg ttacaggacg gattgataag aatgtatcac ca
//
