LOCUS       sequence-68097-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     Collier lab plasmids
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-22b PA K397D/D425K
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtcattatc ctatctcata gcctttttta gaaaagatta aaattttctt gatcccgttg
      121 gtactagtat ccccattctc actaggatta ataatagtgt tttctttagt aacagcatat
      181 acatttacct tataattggg attacttata tataacggta atttatcatt atatttttta
      241 aaatctataa atgtttttcc atcttgccgt aaactagaaa tattcaacat atcatatctg
      301 tcatttataa cttctttaag cccttcagta tcttcaattt ctacaatata acctgataat
      361 atttttctta tatccttatc aatatttaac aataatccct ctgttgacga attaattact
      421 tctctatgag cctccttaac tactgactca tccgccccaa ctgctatgtt atttctatca
      481 taatgaaaac gtttatctct tattaaaata ttcatttttg catttaattt gattttatct
      541 aatacagtat atatgttagt tgcgtttaat tccgctaact gattcttgat attttgagat
      601 gtttgttgat cgaaattaaa atcaaattcg gttatgtctt tcccttgata ttgtaagttt
      661 ccattcggtt cgttaaatcc aaatgctatt ttaagggctt cttttaatgt catatccggt
      721 ttagtcgttt ctaatggatc actaggatta accgccgcta tccgcctttc taccagattt
      781 aaatcttttc cattaaaaat gatacgtgca gttgtttctt gaatttgcgg taacacttca
      841 ctccagttcg agcctgtatc caccctcact cttccatttt caaaattgta tgttgctata
      901 ttcccatata cttgatccgt atctaatctt aattgtttcg ttt
//
