LOCUS       sequence-68103-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Richards CI, Srinivasan R, Xiao C, Mackey ED, Miwa JM, Lester HA
  TITLE     Trafficking of {alpha}4* Nicotinic Receptors Revealed by 
            Superecliptic Phluorin: EFFECTS OF A {beta}4 AMYOTROPHIC LATERAL 
            SCLEROSIS-ASSOCIATED MUTATION AND CHRONIC EXPOSURE TO NICOTINE.
  JOURNAL   J Biol Chem. 2011 Sep 9;286(36):31241-9. Epub 2011 Jul 18.
  PUBMED    21768117
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-09-9"; volume: "286(36)"; pages: "31241-9. Epub 
            2011 Jul 18"
COMMENT     Sequence Label: pCI-neo-alpha4-SEP
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(145..864)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MISKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(188..208)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     803..831
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgtctgctcg aagcattaac cctcactaaa gggaagcggc cgcccgggtc gactctagag
       61 gtaccacgcg tgaattcgcc cttgtgggtg gactgacgag tcgcatggcc ctgtagagac
      121 ctgggagcta ggtgccgcta ttccctattt gtatagttca tccatgccat gtgtaatccc
      181 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      241 aagagtagaa gttgtaaaca ggtaatggtt gtctggtaaa aggacgggcc catcgccaat
      301 tggagtattt tgttgataat ggtctgctag ttgaacgcct ccatcttcaa tgttgtgtct
      361 aattttgaag ttagctttga ttccattctt ttgtttgtct gccatgatgt acacctggtg
      421 atcgttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat
      481 accttttaac tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt
      541 cttgtagttc ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat
      601 ggcactcttg aaaaagtcat gccgtttcat atgatctggg tatcttgaaa agcattgaac
      661 accataagtt aaagtagtga caagtgttgg ccaaggaaca ggtagttttc cagtagtgca
      721 aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga
      781 aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
      841 aagttcttct cctttactga tcataccagc caaccatgga ggcaggaaga gtcccacagt
      901 gcccagaagg cagacaatga tgaacatcc
//
